Efficient generation of functional haploid spermatids from human germline stem cells by three-dimensional-induced system
نویسندگان
چکیده
منابع مشابه
O-9: Generation of Haploid Spermatids with Fertilization and Development Capacity from Human Spermatogonial Stem Cells of Cryptorchid Patients
Background Infertility affects around 15% of couples, and male factors account for 50%. Cryptorchidism is one of the most common causes for azoospermia. Generation of functional spermatids from azoospermia patients is of unusual significance for treating male infertility. It has been recently reported by peers and us that human spermatogonial stem cells (SSCs) can be clearly identified and cult...
متن کاملAB182. Efficient generation of haploid spermatids with Fertilization and development capacity from human spermatogonial stem cells of cryptorchid patients
Generation of functional spermatids from azoospermia patients is of unusual significance in the treatment of male infertility. Here, we report an efficient approach to obtain human functional spermatids from cryptorchid patients. Spermatogonia remained whereas meiotic germ cells were rare in cryptorchid patients. Expression of numerous markers for meiotic and postmeiotic male germ cells was enh...
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BACKGROUND The efficient generation of hematopoietic stem cells (HSCs) from human-induced pluripotent stem cells (iPSCs) holds great promise in personalized transplantation therapies. However, the derivation of functional and transplantable HSCs from iPSCs has had very limited success thus far. METHODS We developed a synthetic 3D hematopoietic niche system comprising nanofibers seeded with bo...
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Generating human podocytes in vitro could offer a unique opportunity to study human diseases. Here, we describe a simple and efficient protocol for obtaining functional podocytes in vitro from human induced pluripotent stem cells. Cells were exposed to a three-step protocol, which induced their differentiation into intermediate mesoderm, then into nephron progenitors and, finally, into mature p...
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ژورنال
عنوان ژورنال: Cell Death & Differentiation
سال: 2018
ISSN: 1350-9047,1476-5403
DOI: 10.1038/s41418-017-0015-1